A Practical Guide to the Histology of the Mouse
[Book Description]
A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year. Complete phenotypic analysis of all of these models can benefit from histologic review of the tissues. This book is aimed at veterinary and medical pathologists who are unfamiliar with mouse tissues and scientists who wish to evaluate their own mouse models. It provides practical guidance on the collection, sampling and analysis of mouse tissue samples in order to maximize the information that can be gained from these tissues. As well as illustrating the normal microscopic anatomy of the mouse, the book also describes and explains the common anatomic variations, artefacts associated with tissue collection and background lesions to help the scientist to distinguish these changes from experimentally- induced lesions. This will be an essential bench-side companion for researchers and practitioners looking for an accessible and well-illustrated guide to mouse pathology. * Written by experienced pathologists and specifically tailored to the needs of scientists and histologists * Full colour throughout * Provides advice on sampling tissues, necropsy and recording data * Includes common anatomic variations, background lesions and artefacts which will help non-experts understand whether histologic variations seen are part of the normal background or related to their experimental manipulation
[Table of Contents]
List of contributors ix
Foreword xi
Preface xiii
About the companion website xv
Chapter 1 Necropsy of the mouse 1 (24)
Lorna Rasmussen
Elizabeth McInnes
1.1 Recording of findings 2 (1)
1.2 Bleeding technique 3 (1)
1.3 Perfusion 3 (1)
1.4 External examination 4 (2)
1.5 Weighing of organs 6 (1)
1.6 Positioning of mouse for necropsy and 6 (3)
removing the skin
1.7 Opening the abdominal cavity and 9 (8)
exposing organs
1.8 Removing the ribcage to expose lungs 17 (3)
and heart
1.9 Removing the brain and spinal cord 20 (2)
1.10 Collecting and fixing tissue samples 22 (3)
References 22 (3)
Chapter 2 Practical approaches to reviewing 25 (18)
and recording pathology data
Cheryl L. Scudamore
2.1 Sample selection and trimming patterns 26 (2)
2.2 Controls 28 (1)
2.3 Standardizing terminology 28 (2)
2.4 Microscopic terminology 30 (3)
2.5 Recording pathology data 33 (2)
2.6 Quantitative versus semiquantitative 35 (2)
analysis
2.7 Semiquantitative techniques 37 (1)
2.8 Quantitative techniques 38 (5)
References 40 (3)
Chapter 3 Gastrointestinal system 43 (20)
Cheryl L. Scudamore
3.1 Background and development 43 (1)
3.2 Oral cavity 43 (3)
3.3 Salivary glands 46 (2)
3.4 Stomach and intestines 48 (8)
3.5 Liver 56 (3)
3.6 Pancreas 59 (4)
References 61 (2)
Chapter 4 Cardiovascular system 63 (12)
Cheryl L. Scudamore
4.1 Background and development 63 (1)
4.2 Sampling techniques and morphometry 63 (2)
4.3 Artefacts 65 (2)
4.4 Anatomy and histology of the heart 67 (2)
4.5 Anatomy and histology of the blood 69 (6)
vessels
References 72 (3)
Chapter 5 Urinary system 75 (12)
Elizabeth McInnes
5.1 Background and development 75 (1)
5.2 Sampling techniques 76 (1)
5.3 Artefacts 76 (1)
5.4 Background lesions 77 (2)
5.5 Anatomy and histology 79 (8)
References 85 (2)
Chapter 6 Reproductive system 87 (22)
Cheryl L. Scudamore
6.1 Background and development of the 87 (1)
male and female reproductive tract
6.2 Female reproductive tract 88 (9)
6.3 Male reproductive tract 97 (12)
References 105 (4)
Chapter 7 Endocrine system 109 (14)
Ian Taylor
7.1 Introduction 109 (1)
7.2 Adrenals 109 (5)
7.3 Pituitary 114 (3)
7.4 Thyroids and parathyroids 117 (6)
References 121 (2)
Chapter 8 Nervous system 123 (26)
Aude Roulois
8.1 Introduction 123 (1)
8.2 Necropsy 123 (3)
8.3 Fixation/perfusion 126 (5)
8.4 Trimming 131 (2)
8.5 Special stains and techniques 133 (4)
8.6 Microscopic examination 137 (1)
8.7 Normal histology-juvenile -- CNS and 137 (4)
PNS
8.8 Artefacts -- CNS and PNS 141 (1)
8.9 Pathological changes and background 142 (4)
pathology -- CNS and PNS
8.10 Acknowledgment 146 (3)
References 146 (2)
Useful website resources 148 (1)
Chapter 9 Lymphoid and haematopoietic system 149 (20)
Ian Taylor
9.1 Introduction 149 (1)
9.2 Lymph nodes 150 (6)
9.3 Spleen 156 (4)
9.4 MALT (mucosa-associated lymphoid 160 (1)
tissue)
9.5 Thymus 161 (3)
9.6 Bone marrow 164 (5)
References 166 (3)
Chapter 10 Integument and adipose tissue 169 (10)
Cheryl L. Scudamore
10.1 Background and development 169 (1)
10.2 Sampling technique 170 (1)
10.3 Anatomy and histology 171 (8)
References 177 (2)
Chapter 11 The respiratory system 179 (16)
Elizabeth McInnes
11.1 Background and development 179 (1)
11.2 Embryology 179 (1)
11.3 Anatomy and histology of the 180 (1)
respiratory system
11.4 Upper respiratory tract 180 (9)
11.5 Lower respiratory tract 189 (6)
References 193 (2)
Chapter 12 Special senses 195 (16)
Cheryl L. Scudamore
12.1 Background and development 195 (1)
12.2 Sampling technique for the eye 195 (2)
12.3 Artefacts 197 (2)
12.4 Anatomy and histology of the eye and 199 (5)
associated glands
12.5 Background and development of the ear 204 (1)
12.6 Sampling technique for the ear and 205 (1)
associated structures
12.7 Anatomy and histology of the ear and 206 (5)
associated glands
References 209 (2)
Chapter 13 Musculoskeletal system 211 (10)
Cheryl L. Scudamore
13.1 Background and development 211 (1)
13.2 Sampling techniques 211 (1)
13.3 Anatomy and histology 212 (9)
References 218 (3)
Index 221
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