Full Description

Summarizes information on autofluorescence of plant secretory cells as a phenomenon and the possibilities of the practical use of light emission. This book is intended for teachers, postgraduate students, and others interested in biological light emission.

Contents List

Autofluorescence of Secretory Cells as a Phenomenon; Secretory Cells; Technique for the Study of Secretory Cell's Autofluorescence; Characteristics of Fluorescing Secretory Cells; Autofluorescence of Specialized Sectory Cells; Secretory Cells in Spore-breeding Plants; Secretory Cell of Seed-breeding Plants; Fluorescence of Secretions and their Individual Components; Spectral Analysis of Excretions and Extracts from Secretory Structures; Fluorescent Substances of Secretions; Autofluorescence of Secretory Cells During their Development; Development of Unicellular Systems; Development of Complex Secretory Structures; Fluorescence of Living Cells at Intercellular Contacts; Pollen-pistil Interactions. Contacts at the Fertilization; Interactions at the Allelopathy. Contacts between Cells from Different Plant Species; Autofluorescence in Cellular Diagnostics; Express-microanalysis of the State and Accumulation of Secondary Metabolites; Cell Viability and Compatibility; Autofluorescence in Diagnostics of Cellular Damage; Diagnostics of Cellular Processes on the Fluorescing Cellular Models; Fluorescence of Cells as Biosensors Bioindicator Reactions; Cytodiagnostics in Botanical Investigation and Education; Individual Components of Secretions as Fluorescence Dyes and Probes; Interactions of Cells with Fluorescent Components of Secretions; Possible Mechanisms of the Emission of the Secretory Products at the Interaction with Living Cells; Conclusion; Appendix 1/ Appendix 2; Color Plate Section.

Table of Contents

Preface                                            v

Introduction                                       vii

Acknowledgement                                    xiii

1. Autofluorescence of Secretory Cells as a        1

Phenomenon

  1.1 Secretory Cells                              2

  1.2 Technique for the Study of the Secretory     5

  Cell's Autofluorescence

  1.3 Characteristics of Fluorescing Secretory     11

  Cells

    1.3.1 Fluorescence of Secretory Cell           12

    1.3.2 Contribution of Individual Components    16

    in the Cellular Fluorescence

    1.3.3 Light Emission of Different Cellular     24

    Compartments and Organisms, which Could

    Live on Plants

  Conclusion                                       31

2. Autofluorescence of Specialized Secretory       32

Cells

  2.1 Secretory Cells in Spore-breeding Plants     46

    2.1.1 Sporangia and Spores                     47

    2.1.2 Gametophytes and Sexual Organs           49

    2.1.3 Hydathodes and Slime-releasing Cells     51

  2.2 Secretory Cells of Seed-breeding Plants      54

    2.2.1 Reproductive Organs                      54

    2.2.2 Secretory Structures in                  69

    Non-reproductive Organs

  Conclusion                                       106

3. Fluorescence of Secretions and their            107

Individual Components

  3.1 Spectral Analysis of Excretions and          107

  Extracts from Secretory Structures

    3.1.1 Crystals                                 108

    3.1.2 Sporopollenin of Microspores             111

    3.1.3 Liquid Excretions                        114

  3.2 Fluorescent Substances of Secretions         123

    3.2.1 Fluorescence of Secondary Metabolites    124

    Found in Secretions

    3.2.2 Changes in the Fluorescence of           143

    Individual Compounds in Secretions

  Conclusion                                       154

4. Autofluorescence of Secretory Cells During      155

their Development

  4.1 Development of Unicellular Systems           155

    4.1.1 Development of Secretory Structures      155

    in Spore-bearing Plants

    4.1.2 Development of Seed-bearing Plants       163

  4.2 Development of Complex Secretory             170

  Structures

    4.2.1 Glandular Structures                     171

  Conclusion                                       183

5. Fluorescence of Living Cells at                 184

Intercellular Contacts

  5.1 Pollen-pistil Interactions. Contacts at      185

  the Fertilization

    5.1.1 Interactions at the Contact              185

    "Pollen-pistil"

    5.1.2 Mechanisms of the Fluorescence           188

    Changes at Pollination

  5.2 Interactions at the Allelopathy. Contacts    194

  between Cells from Different Plant Species

    5.2.1 Pollen-pollen Interactions               194

    5.2.2 Microorganism-plant or Fungi-plant       199

    Relations

    5.2.3 Fluorescence at Modelling of             200

    Allelopathic Relations

    5.2.4 Mechanisms of the Fluorescence Changes   202

  Conclusion                                       205

6 Autofluorescence in Cellular Diagnostics         206

  6.1 Express-microanalysis of the State and       206

  Accumulation of Secondary Metabolites

    6.1.1 Secretory Cells in Medicinal and         207

    Economic Plants

    6.1.2 Pollen State                             210

  6.2 Cell Viability and Compatibility             217

    6.2.1 Pollen Viability                         217

    6.2.2 Self-incompatibility of Pollen           217

  6.3 Autofluorescence in Diagnostics of           219

  Cellular Damage

    6.3.1 Diagnostics of Stress or Ageing          219

    Effects

    6.3.2 Fluorescing Products of Damage           226

  6.4 Diagnostics of Cellular Processes on the     228

  Fluorescing Cellular Models

  6.5 Fluorescence of Cells as Biosensors and      228

  Bioindicator Reactions

  6.6 Cytodiagnostics in Botanical                 230

  Investigation and Education

  Conclusion                                       232

7. Individual Components of Secretions as          233

Fluorescent Dyes and Probes

  7.1 Interaction of Cells with Fluorescent        233

  Components of the Secretions

    7.1.1 Binding with the Cell Wall               234

    7.1.2 Binding with Receptors                   234

    7.1.3 Binding with the Surface Enzymes (ATP    242

    ase and Cholinesterase)

    7.1.4 Binding with Sensors Participating in    243

    Intracellular Regulation of Cyclic AMP

    Concentration

    7.1.5 Binding with DNA-containing Organelles   243

    7.1.6 Binding with Contractile Proteins        249

  7.2 Possible Mechanisms of the Emission of       252

  the Secretory Products at the Interaction

  with Living Cells

    7.2.1 Proteins                                 252

    7.2.2 Lipids                                   253

    7.2.3 Nucleic Acids                            254

    7.2.4 High Molecular and Low Molecular         254

    Secondary Metabolites

  Conclusion                                       255

Conclusion                                         255

Appendix 1: Glossary of Biological Terms           261

Appendix 2: Colour Photographs of Secreting        263

Cells

Bibliography                                       281

Latin Index                                        316

Subject Index                                      323