About this title: Hands-on experts in nanomaterial synthesis and application describe in detail the key experimental techniques currently employed in novel materials synthesis, dynamic cellular imaging, and biological assays. The author's emphasize diverse strategies to synthesize and functionalize the use of nanoparticles for biological applications. Additional chapters focus on the use of biological components (peptides, antibodies, and DNA) to synthesize and organize nanoparticles to be used a building block in larger assemblies. These new materials make it possible to image cellular processes for longer durations, leading to high throughput cellular-based screens for drug discovery, drug delivery, and diagnostic applications. Highlights include overview chapters on quantum dots and DNA nanotechnology, and cutting-edge techniques in the emerging nanobiotachnology arena.

Table Of Contents

Preface

Contributors

Companion CD

       1 Applications of Quantum Dots in Biology: An Overview

             Charles Z. Hotz

       2 Fluoroimmunoassays Using Antibody-Conjugated Quantum Dots

             Ellen R. Goldman, Hedi Mattoussi, George P. Anderson, Igor L. Medintz, and J. Matthew Mauro

       3 Labeling Cell-Surface Proteins Via Antibody Quantum Dot Streptavidin Conjugates

             John N. Mason, Ian D. Tomlinson, Sandra J. Rosenthal, and Randy D. Blakely

       4 Peptide-Conjugated Quantum Dots: Imaging the Angiotensin Type 1 Receptor in Living Cells

             Ian D. Tomlinson, John N. Mason, Randy D. Blakely, and Sandra J. Rosenthal

       5 Quantum Dot-Encoded Beads

             Xiaohu Gao and Shuming Nie

       6 Use of Nanobarcodes?Particles in Bioassays

             R. Griffith Freeman, Paul A. Raju, Scott M. Norton, Ian D. Walton, Patrick C. Smith, Lin He, Michael J. Natan, Michael Y. Sha, and Sharron G. Penn

       7 Assembly and Characterization of Biomolecule-Gold Nanoparticle Conjugates and Their Use in Intracellular Imaging

             Alexander Tkachenko, Huan Xie, Stefan Franzen, and Daniel L. Feldheim

       8 Whole-Blood Immunoassay Facilitated by Gold Nanoshell-Conjugate Antibodies

             Lee R. Hirsch, Naomi J. Halas, and Jennifer L. West

       9 Assays for Selection of Single-Chain Fragment Variable Recombinant Antibodies to Metal Nanoclusters

             Jennifer Edl, Ray Mernaugh, and David W. Wright

10 Surface-Functionalized Nanoparticles for Controlled Drug Delivery

             Sung-Wook Choi, Woo-Sik Kim, and Jung-Hyun Kim

11 Screening of Combinatorial Peptide Libraries for Nanocluster Synthesis

             Joseph M. Slocik and David W. Wright

12 Structural DNA Nanotechnology: An Overview

             Nadrian C. Seeman

13 Nanostructured DNA Templates

             Jeffery L. Coffer, Russell F. Pinizzotto, and Young Gyu Rho

14 Probing DNA Structure With Nanoparticles

             Rahina Mahtab and Catherine J. Murphy

15 Synthetic Nanoscale Elements for Delivery of Materials Into Viable Cells

             Timothy E. McKnight, Anatoli V. Melechko, Michael A. Guillorn, Vladimir I. Merkulov, Douglas H. Lowndes, and Michael L. Simpson

16 Real-Time Cell Dynamics With a Multianalyte Physiometer

             Sven E. Eklund, Eugene Kozlov, Dale E. Taylor, Franz Baudenbacher, and David E. Cliffel

Index