Proteins : Biochemistry and Biotechnology

[BOOK DESCRIPTION]

Proteins Biochemistry and Biotechnology 2e is a definitive source of information for all those interested in protein science, and particularly the commercial production and isolation of specific proteins, and their subsequent utilization for applied purposes in industry and medicine. Fully updated throughout with new or fundamentally revised sections on proteomics as, bioinformatics, protein glycosylation and engineering, well as sections detailing advances in upstream processing and newer protein applications such as enzyme-based biofuel production this new edition has an increased focus on biochemistry to ensure the balance between biochemisty and biotechnology, enhanced with numerous case studies. This second edition is an invaluable text for undergraduates of biochemistry and biotechnology but will also be relevant to students of microbiology, molecular biology, bioinformatics and any branch of the biomedical sciences who require a broad overview of the various medical, diagnostic and industrial uses of proteins. * Provides a comprehensive overview of all aspects of protein biochemisty and protein biotechnology * Includes numerous case studies * Increased focus on protein biochemistry to ensure balance between biochemisty and biotechnology * Includes new section focusing on proteomics as well as sections detailing protein function and enzyme-based biofuel production "With the potential of a standard reference source on the topic, any molecular biotechnologist will profit greatly from having this excellent book. " ( Engineering in Life Sciences , 2004; Vol 5; No. 5) "Few texts would be considered competitors, and none compare favorably." ( Biochemistry and Molecular Education , July/August 2002) "...The book is well written, making it informative and easy to read..." ( The Biochemist , June 2002)

[TABLE OF CONTENTS]

Preface                                            xi
About the Companion Website                        xiii
Chapter 1 Proteins and proteomics                  1   (24)
  1.1 Proteins, an introduction                    1   (1)
  1.2 Genes, genomics and proteomics               2   (10)
  1.3 Bioinformatics                               12  (2)
  1.4 Proteomics: goals and applications           14  (8)
  Further reading                                  22  (3)
Chapter 2 Protein structure and engineering        25  (40)
  2.1 Primary structure                            25  (11)
  2.2 Higher-level structure                       36  (5)
  2.3 Protein classification on the basis of       41  (4)
  structure
  2.4 Protein structural stability                 45  (2)
  2.5 Higher-order structure prediction            47  (1)
  2.6 Protein folding                              48  (2)
  2.7 Intrinsically disordered proteins            50  (1)
  2.8 Protein engineering                          51  (3)
  2.9 Protein post-translational modification      54  (8)
  Further reading                                  62  (3)
Chapter 3 Protein sources                          65  (26)
  3.1 Recombinant versus non-recombinant           65  (2)
  production
  3.2 Approaches to recombinant protein            67  (5)
  production
  3.3 Heterologous protein production in E. coli   72  (5)
  3.4 Heterologous production in bacteria other    77  (1)
  than E. coli
  3.5 Heterologous protein production in yeast     77  (1)
  3.6 Heterologous protein production in fungi     78  (2)
  3.7 Proteins from plants                         80  (4)
  3.8 Animal tissue as a protein source            84  (1)
  3.9 Heterologous protein production in           85  (1)
  transgenic animals
  3.10 Heterologous protein production using       86  (1)
  animal cell culture
  3.11 Insect cell culture systems                 87  (1)
  Further reading                                  88  (3)
Chapter 4 Protein purification and                 91  (50)
characterization
  4.1 Protein detection and quantification         93  (2)
  4.2 Initial recovery of protein                  95  (3)
  4.3 Removal of whole cells and cell debris       98  (5)
  4.4 Concentration                                103 (4)
  4.5 Chromatographic purification                 107 (21)
  4.6 Protein inactivation and stabilization       128 (9)
  4.7 Protein characterization                     137 (2)
  Further reading                                  139 (2)
Chapter 5 Large-scale protein production           141 (36)
  5.1 Upstream processing                          141 (13)
  5.2 Downstream processing                        154 (9)
  5.3 Therapeutic protein production: some         163 (3)
  special issues
  5.4 Range and medical significance of            166 (9)
  impurities potentially present in
  protein-based therapeutic products
  Further reading                                  175 (2)
Chapter 6 Therapeutic proteins: blood products,    177 (28)
vaccines and enzymes
  6.1 Blood products                               177 (7)
  6.2 Anticoagulants                               184 (2)
  6.3 Thrombolytic agents                          186 (3)
  6.4 Additional blood-related products            189 (1)
  6.5 Vaccine technology                           190 (4)
  6.6 Therapeutic enzymes                          194 (8)
  Further reading                                  202 (3)
Chapter 7 Therapeutic antibodies                   205 (28)
  7.1 Antibodies                                   205 (1)
  7.2 IgG structure and activity                   205 (4)
  7.3 Antibody therapeutics: polyclonal            209 (2)
  antibody preparations
  7.4 Antibody therapeutics: monoclonal            211 (9)
  antibodies
  7.5 Therapeutic applications of monoclonal       220 (3)
  antibodies
  7.6 Antibody conjugates                          223 (1)
  7.7 Bispecific antibodies                        224 (1)
  7.8 Antibody fragments                           225 (3)
  7.9 Engineering the antibody glycocomponent      228 (1)
  7.10 Fc fusion proteins                          229 (1)
  Further reading                                  230 (3)
Chapter 8 Hormones and growth factors used         233 (24)
therapeutically
  8.1 Insulin                                      233 (7)
  8.2 Glucagon                                     240 (1)
  8.3 Gonadotrophins                               240 (3)
  8.4 Growth hormone                               243 (3)
  8.5 Erythropoietin                               246 (1)
  8.6 Other hormones                               247 (2)
  8.7 Growth factors                               249 (4)
  Further reading                                  253 (4)
Chapter 9 Interferons, interleukins and tumour     257 (20)
necrosis factors
  9.1 Regulatory factors: cytokines versus         257 (1)
  hormones
  9.2 Interferons                                  258 (6)
  9.3 Interleukins                                 264 (7)
  9.4 Tumour necrosis factors                      271 (3)
  Further reading                                  274 (3)
Chapter 10 Proteins used for analytical purposes   277 (34)
  10.1 The IVD sector                              279 (1)
  10.2 The basis of analyte detection and          280 (1)
  quantification
  10.3 Enzymes as diagnostic/analytical reagents   281 (8)
  10.4 Biosensors                                  289 (6)
  10.5 Antibodies as analytical reagents           295 (14)
  Further reading                                  309 (2)
Chapter 11 Industrial enzymes: an introduction     311 (16)
  11.1 Sales value and manufacturers               313 (1)
  11.2 Sources and engineering                     314 (1)
  11.3 Environmental benefits                      315 (1)
  11.4 Enzyme, detection and quantification        315 (1)
  11.5 Immobilized enzymes                         316 (3)
  11.6 Extremophiles                               319 (5)
  11.7 Enzymes in organic solvents                 324 (1)
  11.8 Industrial enzymes: the future              325 (1)
  Further reading                                  325 (2)
Chapter 12 Industrial enzymes: proteases and       327 (44)
carbohydrases
  12.1 Proteolytic enzymes                         327 (13)
  12.2 Carbohydrases                               340 (27)
  Further reading                                  367 (4)
Chapter 13 Additional industrial enzymes           371 (22)
  13.1 Lipases                                     371 (4)
  13.2 Penicillin acylase                          375 (3)
  13.3 Amino acylase and amino acid production     378 (2)
  13.4 Cyclodextrins and cyclodextrin              380 (2)
  glycosyltransferase
  13.5 Enzymes and animal nutrition                382 (5)
  13.6 Enzymes in molecular biology                387 (3)
  Further reading                                  390 (3)
Chapter 14 Non-catalytic industrial proteins       393 (24)
  14.1 Functional properties of proteins           393 (4)
  14.2 Milk and milk proteins                      397 (11)
  14.3 Animal-derived proteins                     408 (3)
  14.4 Plant-derived proteins                      411 (1)
  14.5 Sweet and taste-modifying proteins          412 (2)
  Further reading                                  414 (3)
Index                                              417